ABSTRACT
<p><b>OBJECTIVE</b>To develop a high-performance liquid chromatography (HPLC) method for the simultaneous determination of rutin, hyperoside, isoquercetin, astragulin, quercetin, and kaempferol in Apocynum venetum and its extracts.</p><p><b>METHOD</b>The separation was carried out on a Shim pack ODS (4.6 mm x 250 mm, 5 microm) colum eluted with in mobile phases of water containing 0.2% phosphoric acid and acetonitrile containing 0.2% phosphoric acid in acetonitrile gradient mode. The column temperature was 40 degrees C, and the flow rate was 1.0 mL x min(-1). The detection wavelength was set at 360 nm.</p><p><b>RESULT</b>The good seperation of six flavonoids was achieved within 40 min, with the relative standard deviations (RSD) of intra- and inter-day precision < or = 2.0%. Calibration curves of rutin, hyperoside, isoquercetin, astragulin, quercetin, and kaempferol showed good linear relationship (R2 > 0.999 7, n = 6). The average recoveries of the six flavonoids were within 97.30% - 105.8% (RSD 2.6%). Three batches of A. venetum and 2 batches of its extracts were determined.</p><p><b>CONCLUSION</b>The developed method is simple, accurate, and repeatable, and can be readily used as a powerful tool for the quality control of A. venetum and its extracts.</p>
Subject(s)
Apocynum , Chemistry , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Chemistry , Flavonoids , Quality Control , Reproducibility of ResultsABSTRACT
AIM: To investigate the effect of autologous bone marrow mononuclear cells (BM-MNCs) local transplantation (BM-LT) on a rabbit model of hindlimb ischemia. METHODS: After unilateral femoral artery and its branches were surgically ligated and excised in rabbits (n=14) 1 hour or so, autologous BM-MNCs were injected into the ischemic skeletal muscles in 7 rabbits, and phosphate buffered saline for another 7 ones as control. Hemodynamics parameters were measured at day 0, day 7 and day 28, respectively. On day 28, the animals were sacrificed in groups for pathological analysis. RESULTS: Compared with control group, color Doppler flow image in BM-LT group was recovered significantly, and its' systolic peak velocity and end-diastolic velocity were also improved (P
ABSTRACT
AIM: To investigate the effect of Fas ligand on reendothelialization in the rat carotid artery with balloon withdrawl injury. METHODS: Balloon withdrawal injury was used to establish the denuded carotid artery rat model in twelve SD rats and then six SD rats were treated with ?-gal virus supernatant (?-gal group), and the others were treated with FasL virus supernatant (FasL group). After fourteen days, Evans blue dying was conducted via injection through rat tail vein at 30 min before the animals were killed, then fixation perfusion in situ was performed with 10% formalin and specimens were obtained. Histologic observation and morphometric analysis were made. RESULTS: The reenothelialization area (RA) of arterial balloon injury and the ratio of the reenothelialization area to the total area ((RA/TA)) significantly increased in FasL group compared with those in ?-gal group (P
ABSTRACT
Objective To explore the expansion and differentiation of bone marrow endothelial progenitor cells in vitro. Methods Rabbit bone marrow mononuclear cells were isolated by gradient density centrifugation.Attached cells were cultured and in endothelial cell growth supplement(ECGS) from bovine pituitary.Cell multiplication was observed,and their biological characteristics were detected. Results The number of attached cells increased 5～8 fold with each expanded.Cobblestone like structure was observed when the cells lined in single layer.Expanded cells expressed von Willebrand factor(vWF) and could ingest Dil acLDL. ConclusionEndothelial progenitor cells,which exist within adult bone marrow,have the potential to be expanded and differentiated when stimulated by ECGS in vitro.